The Internal Positive Control (IPC) is spiked into samples before qPCR assay to confirm the absence of target sequence, inhibition or a reaction set-up/cycling error.
Positive controls
Positive controls allow you to detect inhibited amplification reactions due to the carryover of reagents used for the isolation of nucleic acids. Other causes of false-negative results include target nucleic acid degradation, sample processing errors and thermocycler malfunction.
Benefits :
- Specific: Does not interfere with target amplification and avoid amplification of endogenous genes
- Sensitive: Compatible with low copy templates and available with dark quencher for maximal signal-to-noise ratio
- Designed for multiplex assays: Detected in the Yakima Yellow®/VIC®/and JOE channel. Validated in duplex assays with the target in the FAM channel
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